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Finally, the heterologous production of GSLs in Nicotiana benthamiana leaves revealed the GSL sulfation as a bottleneck in GSL biosynthesis

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Materials and Methods Cell Culture U-87 MG cells were maintained in Eagles Minimum Essential Medium supplemented with 10% FBS and 1x pen-strep

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Recently, a novel pathogenetic model has been proposed which postulated that changes in the local metabolic environment (pH, salt) may play a key role in the development of AS by induction of a Th17 pro-inflammatory phenotype through activation of glycosphingolipid sensors (encoded by the GPR genes in particularly GPR65 ) and serum and glucocorticoid-regulated kinase-1 (SGK1) ( Figure 4 - 11 )